https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Clinicopathological significance of nerves in esophageal cancer https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37859 P = 0.04). The surrounding or invasion of nerves by cancer cells (perineural invasion) was detected in 12% of esophageal cancers and was associated with reduced survival (P = 0.04). Nerves were found to express the following receptors for nerve growth factor (NGF): neurotrophic receptor tyrosine kinase 1 and nerve growth factor receptor. An association was suggested between high production of NGF by cancer cells and the presence of nerves (P = 0.02). In vitro, NGF production in esophageal cancer cells was shown by Western blot, and esophageal cancer cells were able to induce neurite outgrowth in the PC12 neuronal cells. The neurotrophic activity of esophageal cancer cells was inhibited by anti-NGF blocking antibodies. Together, these data suggest that innervation is a feature in esophageal cancers that may be driven by cancer cell-released NGF.]]> Wed 17 Nov 2021 16:31:21 AEDT ]]> Neurotrophin receptors TrkA, p75NTR, and sortilin are increased and targetable in thyroid cancer https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36867 NTR, and the proneurotrophin receptor sortilin were analyzed with immunohistochemistry in a cohort of thyroid cancers (n = 128) and compared with adenomas and normal thyroid tissues (n = 62). TrkA was detected in 20% of thyroid cancers, compared with none of the benign samples (P = 0.0007). TrkA expression was independent of histologic subtypes but associated with lymph node metastasis (P = 0.0148), suggesting the involvement of TrkA in tumor invasiveness. Nerves in the tumor microenvironment were positive for TrkA. p75NTR was overexpressed in anaplastic thyroid cancers compared with papillary and follicular subtypes (P < 0.0001). Sortilin was overexpressed in thyroid cancers compared with benign thyroid tissues (P < 0.0001). Neurotrophin receptor expression was confirmed in a panel of thyroid cancer cell lines at the mRNA and protein levels. Functional investigations using the anaplastic thyroid cancer cell line CAL-62 found that siRNA against TrkA, p75NTR, and sortilin decreased cell survival and cell migration through decreased SRC and ERK activation. Together, these data reveal TrkA, p75NTR, and sortilin as potential therapeutic targets in thyroid cancer.]]> Wed 09 Feb 2022 15:52:42 AEDT ]]> The membrane protein sortilin can be targeted to inhibit pancreatic cancer cell invasion https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37858 P = 0.0014). Sortilin inhibition by siRNA and the pharmacologic inhibitor AF38469 strongly reduced the adhesion and invasion of pancreatic cancer cells without affecting cell survival and viability. Sortilin inhibition also decreased the phosphorylation of the focal adhesion kinase in Tyr925. Together, these data show that sortilin contributes to pancreatic cancer invasion and could eventually be targeted in therapy.]]> Thu 24 Mar 2022 11:34:45 AEDT ]]> IL-6 drives neutrophil-mediated pulmonary inflammation associated with bacteremia in murine models of colitis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34597 Thu 13 Jan 2022 10:31:31 AEDT ]]> Alveolar macrophages stimulate enhanced cytokine production by pulmonary CD4⁺ T-lymphocytes in an exacerbation of murine chronic asthma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11339 Sat 24 Mar 2018 08:08:17 AEDT ]]> ProNGF correlates with Gleason score and is a potential driver of nerve infiltration in prostate cancer https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19564 Sat 24 Mar 2018 07:58:25 AEDT ]]> Deletion of Cd151 results in a strain-dependent glomerular disease due to severe alterations of the glomerular basement membrane https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5279 Sat 24 Mar 2018 07:46:27 AEDT ]]>